Ugaz, V. M., & Priye, A., & Hassan, Y. A. (2012, June), DNA to Go: A Do-it-Yourself PCR Thermocycler Lab  Paper presented at 2012 ASEE Annual Conference & Exposition, San Antonio, Texas. 10.18260/1-2--21231

\bibitem{asee_peer_21231}
  Ugaz, V. M., \& Priye, A., \& Hassan, Y. A. (2012, June), \emph{DNA to Go: A Do-it-Yourself PCR Thermocycler Lab}
  Paper presented at 2012 ASEE Annual Conference \& Exposition, San Antonio, Texas.
  10.18260/1-2--21231

Victor M. Ugaz, Aashish Priye, and Yassin A. Hassan.  "DNA to Go: A Do-it-Yourself PCR Thermocycler Lab".  2012 ASEE Annual Conference & Exposition, San Antonio, Texas, 2012, June.  ASEE Conferences, 2012.  https://strategy.asee.org/21231 Internet.  17 Jul, 2024

\bibitem{asee_peer_21231}
  Victor M. Ugaz, Aashish Priye, and Yassin A. Hassan.
  "DNA to Go: A Do-it-Yourself PCR Thermocycler Lab".
  \emph{2012 ASEE Annual Conference \& Exposition, San Antonio, Texas, 2012, June}.
  ASEE Conferences, 2012.
  https://strategy.asee.org/21231 Internet.  17 Jul, 2024

@INPROCEEDINGS{asee_peer_21231
author = "Victor M. Ugaz, Aashish Priye, and Yassin A. Hassan"
title = "DNA to Go: A Do-it-Yourself PCR Thermocycler Lab"
booktitle = "2012 ASEE Annual Conference \& Exposition"
year = "2012"
month = "June"
address = "San Antonio, Texas"
publisher = "ASEE Conferences"
note = {https://strategy.asee.org/21231}
number = {10.18260/1-2--21231}
}

TY  - CPAPER
AB  -                DNA to Go: A Do-it-Yourself PCR Thermocycler LabThere is currently a need for innovative undergraduate educational experiences that unify andreinforce fundamental principles at the interface between physics, molecular biology, and thechemical sciences. These experiences also empower students by helping them recognize how thisknowledge can be applied to develop new products and technologies that benefit society. Thispresentation describes our efforts to address this need by creating innovative hands-on labactivities that introduce chemical engineering students to molecular biology by challenging themto harness natural convection phenomena to perform DNA replication via the polymerase chainreaction (PCR).Experimentally, we have constructed convective PCR stations incorporating a simple design forloading and mounting the cylindrical PCR reactor between independently controlled thermalplates. Each station independently interfaces with a Windows-based PC via a USB connection,and is operated by a custom designed software package that enables temperature profiles to beeasily input and monitored. A motion analysis microscope enables flow patterns inside theconvective PCR reactors to be directly visualized. We have also developed undergraduate coursemodules focused on modeling the problem of thermal convection in a fluid layer heated frombelow (the Rayleigh-Bénard problem) in the context of geometries that could be used to designlava lamps. These materials directly tie into our core transport sequence core transport sequencecourses. After the fundamental problem is introduced and connected with the core coursematerial, the students are walked through a hands-on CFD exercise, then assigned a problem thatgives them an opportunity to explore the effects of varying parameters in the model. Initialfeedback has been very positive, as the computer simulations seem to excite student interestbecause they can actually “see” what they have been learning in the lecture. These capabilitiesuniquely enable us to connect the theoretical/computational, experimental, and biochemicalreaction into a unified experience.
AU  - Victor M. Ugaz
AU  - Aashish Priye
AU  - Yassin A. Hassan
CY  - San Antonio, Texas
DA  - 2012/06/10
PB  - ASEE Conferences
TI  - DNA to Go: A Do-it-Yourself PCR Thermocycler Lab
UR  - https://strategy.asee.org/21231
DO  - 10.18260/1-2--21231
ER  -